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dapi solution (1 mg/ml)  (Thermo Fisher)


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    Thermo Fisher dapi solution (1 mg/ml)

    Dapi Solution (1 Mg/Ml), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+solution(1+mg/pmc11548877-26-4-9
    Average 90 stars, based on 1 article reviews
    dapi solution (1 mg/ml) - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "FABP4-mediated lipid accumulation and lipolysis in tumor-associated macrophages promote breast cancer metastasis"

    Article Title: FABP4-mediated lipid accumulation and lipolysis in tumor-associated macrophages promote breast cancer metastasis

    Journal: eLife

    doi: 10.7554/eLife.101221


    Figure Legend Snippet:

    Techniques Used: Concentration Assay, Staining, Saline, SYBR Green Assay, Red Blood Cell Lysis, Cell Culture, XF Assay, Reverse Transcription, Polymer, Plasmid Preparation, Blocking Assay, Enzyme-linked Immunosorbent Assay, Sequencing, Transfection, Recombinant, Microarray, Expressing, Derivative Assay, Real-time Polymerase Chain Reaction, Software, Sterility, Migration, Pore Size, Flow Cytometry, Quantitative RT-PCR, Imaging, Microscopy, Transmission Assay, Electron Microscopy

    Related Articles

    Incubation:

    Article Title: The dorsal and ventral hippocampus contribute differentially to spatial working memory and spatial coding in the prefrontal cortex
    Article Snippet: To enhance the visibility of GFP expression, brain sections were incubated overnight with a primary GFP antibody (rabbit anti-GFP, 1:1000, ThermoFisher Scientific, Waltham, USA) in carrier solution (1% horse serum, 0.5% Triton X-100% and 0.2% BSA in PBS) followed by overnight incubation with a secondary green fluorescent antibody (anti-rabbit 488 Alexa Fluor, 1:750, ThermoFisher Scientific, Waltham, USA) in carrier solution. .. To visualize cell bodies, the sections were then incubated in a DAPI solution (1 mg/ml in PBS; ThermoFisher Scientific, Waltham, USA) for 5 min. .. Brain sections were mounted on microscope slides to verify electrode placements and virus expression with a confocal microscope (Eclipse 90i, Nikon, Minato, Japan).

    Article Title: Transcriptional stress induces the overexpression of novel lncRNAs that regulate the BRCA1 locus
    Article Snippet: .. The next day samples were washed once with 1x PBS and incubated with DAPI solution (ThermoFisher, D1306; stock 5 mg/ml diluted 1:20000 in 1x PBS) for 30 min at 37°C. ..

    Article Title: Lumen expansion is initially driven by apical actin polymerization followed by osmotic pressure in a human epiblast model
    Article Snippet: .. After washing three times with 0.1% Triton X-100 in DPBS, DAPI (1:1000 dilution; 5 mg/ml stock solution, Thermo Fisher Scientific) and Alexa Fluor 488 Phalloidin (1:80 dilution; Invitrogen A12379) or Alexa Fluor 555 Phalloidin (1:200 dilution; Invitrogen A34055) were incubated for 1.5 hr to stain the nucleus and F-actin along with appropriate secondary antibodies, such as goat anti-Rabbit IgG AF647 (Invitrogen, 1:200 dilution) and goat anti-mouse IgG1 AF555 (Invitrogen, 1:200 dilution). .. Following three washes with 0.1% Triton X-100 in DPBS, ProLong Gold antifade reagent (Life Technologies) was applied to minimize photobleaching.

    Article Title: The dorsal and ventral hippocampus contribute differentially to spatial working memory and spatial coding in the prefrontal cortex.
    Article Snippet: To enhance the visibility of GFP expression, brain sections were incubated overnight with a primary GFP antibody (rabbit anti-GFP, 1:1000, ThermoFisher Scientific, Waltham, USA) in carrier solution (1% horse serum, 0.5% Triton X-100% and 0.2% BSA in PBS) followed by overnight incubation with a secondary green fluorescent antibody (anti-rabbit 488 Alexa Fluor, 1:750, ThermoFisher Scientific, Waltham, USA) in carrier solution. .. To visualize cell bodies, the sections were then incubated in a DAPI solution (1 mg/ml in PBS; ThermoFisher Scientific, Waltham, USA) for 5 min. .. Brain sections were mounted on microscope slides to verify electrode placements and virus expression with a confocal microscope (Eclipse 90i, Nikon, Minato, Japan).

    Article Title: Identification of a vimentin-expressing α-cell phenotype in CF and normal pancreas
    Article Snippet: .. Subsequently, slides were washed three times in PBS for 5 min on a rotating platform and incubated with 4′,6-diamidino-2-phenylindole (DAPI) solution (0.1 mg/mL in PBS) (Thermo Fisher, UK) for 20 min at room temperature in darkness. ..

    Polymer:

    Article Title: Persistent glycolysis defines the foreign body response to polymeric implants
    Article Snippet: .. 0.5M EDTA (pH 08.0 ± 0.1; Corning 46-034-CI) 1M HEPES (Gibco 15630-080) 1X Antibody Diluent/Block (Akoya Biosciences ARD1001EA) 1X DPBS (Corning 20-031-CV) 1X OPAL TM Polymer HRP MS+RB (Akoya Biosciences ARH1001EA) 1X RPMI 1640 + L-Glutamine (300 mgL - ) (Corning 10-040-CV) 1X Plus Manual Amplification Diluent (Akoya Biosciences FP1498) 10% Neutral Buffered Formalin (VWR 16004-128) Alexa Fluor® 488 Conjugation Kit (Fast) – Lightning-Link ® (ABCAM AB236552) Alexa Fluor® 700 Conjugation Kit (Fast) – Lightning-Link ® (ABCAM AB269824) AR6 Antigen Retrieval Buffer (1-5% w/w Sodium Citrate; Akoya Biosciences AR600250ML) AR9 Antigen Retrieval Buffer (1-2.5% TRIS w/w, <1% EDTA w/w; Akoya Biosciences AR900259ML) BD Horizon TM Fixable Viability Stain 575V (BD Biosciences 565694) BSA (Heat shock fraction; Sigma-Aldrich A7906-50G) Collagenase A (Millipore-Sigma 10103586001) Chloroform (Fisher Scientific AC423555000) DAPI (1 mg mL −1 stock solution; Thermo Fisher Scientific D1306) DMSO (Sigma-Aldrich 67-68-5) DNase I (Millipore-Sigma 10104159001) DyLight® 405 Conjugation Kit (Fast) – Lightning-Link ® (ABCAM AB201798) Ethanol (Commercial Alchohols, P016EAAN) FBS (Heat inactivated, Corning 35-011-CV) FoxP3 Fixation/Permeabilization Buffer (BioLegend 421403) FoxP3 Permeabilization Solution (BioLegend 421403) Harringtonine (ABCAM AB141941) Histoplast TM PE (Thermo Fisher Scientific 22900700) Masson’s Trichrome Staining Kit (Polysciences Inc 25088-1) MitoTracker TM Red CMXRos Dye, for flow cytometry (Thermo Fisher Scientific M46752) Monocyte Blocker (BioLegend 426102) Mowiol® 4-88 (Fisher Scientific 9002-89-5) Oligomycin-A (ABCAM AB143423) Omni International 1.4 mm Ceramic Bead (Cole-Parmer® 19-645-3) PE-Cy7® Conjugation Kit – Lightning-Link ® (ABCAM AB102903) PE-Texas Red Conjugation Kit – Lightning-Link ® (ABCAM AB269899) Permount® Mounting Medium (Fisher Chemical SP15-500) Polydimethylsiloxane (PDMS; SYLGARD 184; Dow Chemical GMID 04019862) Polypropylene (PP; isotactic, avg. .. MW 220 000; Polysciences Inc. 06536) Puromycin (Sigma-Aldrich P8833) Precision Count Beads TM (BioLegend 424902) Rainbow Calibration Particles, 8 peaks (3.0-3.4 μm, BioLegend 422903) RNEasy Minikit Plus (Qiagen 74004) SsoAdvanced Universal SYBR Green Supermix (Bio-Rad 1725274) SuperScript IV VILO Master Mix (Thermo Fisher Scientific 11756050) Tris-HCl Buffered Saline + Tween®20 (TBST; 25 mM TRIS-HCl (pH 7.5), 150 mM NaCl, 0.05% Tween®20) TRIzol TM Reagent (Thiocyanic acid 25-40% w/w, Phenol 30-60% w/w, Ammonium thiocyanate 7-13% w/w; Invitrogen 15596018) TruStain FcX (BioLegend 101319) Ultracomp eBeads TM (Thermo Fisher Scientific, 01222242) Ultra-high MW polyethylene (UHMW-PE; avg.

    Amplification:

    Article Title: Persistent glycolysis defines the foreign body response to polymeric implants
    Article Snippet: .. 0.5M EDTA (pH 08.0 ± 0.1; Corning 46-034-CI) 1M HEPES (Gibco 15630-080) 1X Antibody Diluent/Block (Akoya Biosciences ARD1001EA) 1X DPBS (Corning 20-031-CV) 1X OPAL TM Polymer HRP MS+RB (Akoya Biosciences ARH1001EA) 1X RPMI 1640 + L-Glutamine (300 mgL - ) (Corning 10-040-CV) 1X Plus Manual Amplification Diluent (Akoya Biosciences FP1498) 10% Neutral Buffered Formalin (VWR 16004-128) Alexa Fluor® 488 Conjugation Kit (Fast) – Lightning-Link ® (ABCAM AB236552) Alexa Fluor® 700 Conjugation Kit (Fast) – Lightning-Link ® (ABCAM AB269824) AR6 Antigen Retrieval Buffer (1-5% w/w Sodium Citrate; Akoya Biosciences AR600250ML) AR9 Antigen Retrieval Buffer (1-2.5% TRIS w/w, <1% EDTA w/w; Akoya Biosciences AR900259ML) BD Horizon TM Fixable Viability Stain 575V (BD Biosciences 565694) BSA (Heat shock fraction; Sigma-Aldrich A7906-50G) Collagenase A (Millipore-Sigma 10103586001) Chloroform (Fisher Scientific AC423555000) DAPI (1 mg mL −1 stock solution; Thermo Fisher Scientific D1306) DMSO (Sigma-Aldrich 67-68-5) DNase I (Millipore-Sigma 10104159001) DyLight® 405 Conjugation Kit (Fast) – Lightning-Link ® (ABCAM AB201798) Ethanol (Commercial Alchohols, P016EAAN) FBS (Heat inactivated, Corning 35-011-CV) FoxP3 Fixation/Permeabilization Buffer (BioLegend 421403) FoxP3 Permeabilization Solution (BioLegend 421403) Harringtonine (ABCAM AB141941) Histoplast TM PE (Thermo Fisher Scientific 22900700) Masson’s Trichrome Staining Kit (Polysciences Inc 25088-1) MitoTracker TM Red CMXRos Dye, for flow cytometry (Thermo Fisher Scientific M46752) Monocyte Blocker (BioLegend 426102) Mowiol® 4-88 (Fisher Scientific 9002-89-5) Oligomycin-A (ABCAM AB143423) Omni International 1.4 mm Ceramic Bead (Cole-Parmer® 19-645-3) PE-Cy7® Conjugation Kit – Lightning-Link ® (ABCAM AB102903) PE-Texas Red Conjugation Kit – Lightning-Link ® (ABCAM AB269899) Permount® Mounting Medium (Fisher Chemical SP15-500) Polydimethylsiloxane (PDMS; SYLGARD 184; Dow Chemical GMID 04019862) Polypropylene (PP; isotactic, avg. .. MW 220 000; Polysciences Inc. 06536) Puromycin (Sigma-Aldrich P8833) Precision Count Beads TM (BioLegend 424902) Rainbow Calibration Particles, 8 peaks (3.0-3.4 μm, BioLegend 422903) RNEasy Minikit Plus (Qiagen 74004) SsoAdvanced Universal SYBR Green Supermix (Bio-Rad 1725274) SuperScript IV VILO Master Mix (Thermo Fisher Scientific 11756050) Tris-HCl Buffered Saline + Tween®20 (TBST; 25 mM TRIS-HCl (pH 7.5), 150 mM NaCl, 0.05% Tween®20) TRIzol TM Reagent (Thiocyanic acid 25-40% w/w, Phenol 30-60% w/w, Ammonium thiocyanate 7-13% w/w; Invitrogen 15596018) TruStain FcX (BioLegend 101319) Ultracomp eBeads TM (Thermo Fisher Scientific, 01222242) Ultra-high MW polyethylene (UHMW-PE; avg.

    Conjugation Assay:

    Article Title: Persistent glycolysis defines the foreign body response to polymeric implants
    Article Snippet: .. 0.5M EDTA (pH 08.0 ± 0.1; Corning 46-034-CI) 1M HEPES (Gibco 15630-080) 1X Antibody Diluent/Block (Akoya Biosciences ARD1001EA) 1X DPBS (Corning 20-031-CV) 1X OPAL TM Polymer HRP MS+RB (Akoya Biosciences ARH1001EA) 1X RPMI 1640 + L-Glutamine (300 mgL - ) (Corning 10-040-CV) 1X Plus Manual Amplification Diluent (Akoya Biosciences FP1498) 10% Neutral Buffered Formalin (VWR 16004-128) Alexa Fluor® 488 Conjugation Kit (Fast) – Lightning-Link ® (ABCAM AB236552) Alexa Fluor® 700 Conjugation Kit (Fast) – Lightning-Link ® (ABCAM AB269824) AR6 Antigen Retrieval Buffer (1-5% w/w Sodium Citrate; Akoya Biosciences AR600250ML) AR9 Antigen Retrieval Buffer (1-2.5% TRIS w/w, <1% EDTA w/w; Akoya Biosciences AR900259ML) BD Horizon TM Fixable Viability Stain 575V (BD Biosciences 565694) BSA (Heat shock fraction; Sigma-Aldrich A7906-50G) Collagenase A (Millipore-Sigma 10103586001) Chloroform (Fisher Scientific AC423555000) DAPI (1 mg mL −1 stock solution; Thermo Fisher Scientific D1306) DMSO (Sigma-Aldrich 67-68-5) DNase I (Millipore-Sigma 10104159001) DyLight® 405 Conjugation Kit (Fast) – Lightning-Link ® (ABCAM AB201798) Ethanol (Commercial Alchohols, P016EAAN) FBS (Heat inactivated, Corning 35-011-CV) FoxP3 Fixation/Permeabilization Buffer (BioLegend 421403) FoxP3 Permeabilization Solution (BioLegend 421403) Harringtonine (ABCAM AB141941) Histoplast TM PE (Thermo Fisher Scientific 22900700) Masson’s Trichrome Staining Kit (Polysciences Inc 25088-1) MitoTracker TM Red CMXRos Dye, for flow cytometry (Thermo Fisher Scientific M46752) Monocyte Blocker (BioLegend 426102) Mowiol® 4-88 (Fisher Scientific 9002-89-5) Oligomycin-A (ABCAM AB143423) Omni International 1.4 mm Ceramic Bead (Cole-Parmer® 19-645-3) PE-Cy7® Conjugation Kit – Lightning-Link ® (ABCAM AB102903) PE-Texas Red Conjugation Kit – Lightning-Link ® (ABCAM AB269899) Permount® Mounting Medium (Fisher Chemical SP15-500) Polydimethylsiloxane (PDMS; SYLGARD 184; Dow Chemical GMID 04019862) Polypropylene (PP; isotactic, avg. .. MW 220 000; Polysciences Inc. 06536) Puromycin (Sigma-Aldrich P8833) Precision Count Beads TM (BioLegend 424902) Rainbow Calibration Particles, 8 peaks (3.0-3.4 μm, BioLegend 422903) RNEasy Minikit Plus (Qiagen 74004) SsoAdvanced Universal SYBR Green Supermix (Bio-Rad 1725274) SuperScript IV VILO Master Mix (Thermo Fisher Scientific 11756050) Tris-HCl Buffered Saline + Tween®20 (TBST; 25 mM TRIS-HCl (pH 7.5), 150 mM NaCl, 0.05% Tween®20) TRIzol TM Reagent (Thiocyanic acid 25-40% w/w, Phenol 30-60% w/w, Ammonium thiocyanate 7-13% w/w; Invitrogen 15596018) TruStain FcX (BioLegend 101319) Ultracomp eBeads TM (Thermo Fisher Scientific, 01222242) Ultra-high MW polyethylene (UHMW-PE; avg.

    Staining:

    Article Title: Persistent glycolysis defines the foreign body response to polymeric implants
    Article Snippet: .. 0.5M EDTA (pH 08.0 ± 0.1; Corning 46-034-CI) 1M HEPES (Gibco 15630-080) 1X Antibody Diluent/Block (Akoya Biosciences ARD1001EA) 1X DPBS (Corning 20-031-CV) 1X OPAL TM Polymer HRP MS+RB (Akoya Biosciences ARH1001EA) 1X RPMI 1640 + L-Glutamine (300 mgL - ) (Corning 10-040-CV) 1X Plus Manual Amplification Diluent (Akoya Biosciences FP1498) 10% Neutral Buffered Formalin (VWR 16004-128) Alexa Fluor® 488 Conjugation Kit (Fast) – Lightning-Link ® (ABCAM AB236552) Alexa Fluor® 700 Conjugation Kit (Fast) – Lightning-Link ® (ABCAM AB269824) AR6 Antigen Retrieval Buffer (1-5% w/w Sodium Citrate; Akoya Biosciences AR600250ML) AR9 Antigen Retrieval Buffer (1-2.5% TRIS w/w, <1% EDTA w/w; Akoya Biosciences AR900259ML) BD Horizon TM Fixable Viability Stain 575V (BD Biosciences 565694) BSA (Heat shock fraction; Sigma-Aldrich A7906-50G) Collagenase A (Millipore-Sigma 10103586001) Chloroform (Fisher Scientific AC423555000) DAPI (1 mg mL −1 stock solution; Thermo Fisher Scientific D1306) DMSO (Sigma-Aldrich 67-68-5) DNase I (Millipore-Sigma 10104159001) DyLight® 405 Conjugation Kit (Fast) – Lightning-Link ® (ABCAM AB201798) Ethanol (Commercial Alchohols, P016EAAN) FBS (Heat inactivated, Corning 35-011-CV) FoxP3 Fixation/Permeabilization Buffer (BioLegend 421403) FoxP3 Permeabilization Solution (BioLegend 421403) Harringtonine (ABCAM AB141941) Histoplast TM PE (Thermo Fisher Scientific 22900700) Masson’s Trichrome Staining Kit (Polysciences Inc 25088-1) MitoTracker TM Red CMXRos Dye, for flow cytometry (Thermo Fisher Scientific M46752) Monocyte Blocker (BioLegend 426102) Mowiol® 4-88 (Fisher Scientific 9002-89-5) Oligomycin-A (ABCAM AB143423) Omni International 1.4 mm Ceramic Bead (Cole-Parmer® 19-645-3) PE-Cy7® Conjugation Kit – Lightning-Link ® (ABCAM AB102903) PE-Texas Red Conjugation Kit – Lightning-Link ® (ABCAM AB269899) Permount® Mounting Medium (Fisher Chemical SP15-500) Polydimethylsiloxane (PDMS; SYLGARD 184; Dow Chemical GMID 04019862) Polypropylene (PP; isotactic, avg. .. MW 220 000; Polysciences Inc. 06536) Puromycin (Sigma-Aldrich P8833) Precision Count Beads TM (BioLegend 424902) Rainbow Calibration Particles, 8 peaks (3.0-3.4 μm, BioLegend 422903) RNEasy Minikit Plus (Qiagen 74004) SsoAdvanced Universal SYBR Green Supermix (Bio-Rad 1725274) SuperScript IV VILO Master Mix (Thermo Fisher Scientific 11756050) Tris-HCl Buffered Saline + Tween®20 (TBST; 25 mM TRIS-HCl (pH 7.5), 150 mM NaCl, 0.05% Tween®20) TRIzol TM Reagent (Thiocyanic acid 25-40% w/w, Phenol 30-60% w/w, Ammonium thiocyanate 7-13% w/w; Invitrogen 15596018) TruStain FcX (BioLegend 101319) Ultracomp eBeads TM (Thermo Fisher Scientific, 01222242) Ultra-high MW polyethylene (UHMW-PE; avg.

    Article Title: Impact of extreme weather events on pelagic cyanobacterial communities: an in situ mesocosm study in the Gulf of Finland
    Article Snippet: Cyanobacteria play a critical role in regulating carbon, nitrogen, and phosphorus cycles in the Baltic Sea, seasonally forming extensive blooms under nitrogen-limited conditions in summer.. Understanding their responses to environmental disturbances is crucial in the Baltic Sea where increasing and persistent surface water temperature anomalies were observed over the past two decades.. During a 17-day mesocosm experiment in the southwestern Finnish archipelago (Gulf of Finland) designed to investigate microbial community dynamics under varying nutrient conditions, the unexpected occurrence of a natural disturbance event created a unique opportunity to assess the effects of pronounced physical changes on cyanobacterial dynamics.

    Article Title: Lumen expansion is initially driven by apical actin polymerization followed by osmotic pressure in a human epiblast model
    Article Snippet: .. After washing three times with 0.1% Triton X-100 in DPBS, DAPI (1:1000 dilution; 5 mg/ml stock solution, Thermo Fisher Scientific) and Alexa Fluor 488 Phalloidin (1:80 dilution; Invitrogen A12379) or Alexa Fluor 555 Phalloidin (1:200 dilution; Invitrogen A34055) were incubated for 1.5 hr to stain the nucleus and F-actin along with appropriate secondary antibodies, such as goat anti-Rabbit IgG AF647 (Invitrogen, 1:200 dilution) and goat anti-mouse IgG1 AF555 (Invitrogen, 1:200 dilution). .. Following three washes with 0.1% Triton X-100 in DPBS, ProLong Gold antifade reagent (Life Technologies) was applied to minimize photobleaching.

    Article Title: Obesity alters the fitness of peritumoral adipose tissue, exacerbating tumor invasiveness in renal cancer through the induction of ADAM12 and CYP1B1
    Article Snippet: The Leica DMi8 microscope digital camera system and the Leica Application Suite LAS X Imaging Software (Leica Microsystems GmbH) were used to measure the diameter of the spheroids following 2, 4, 7, 9, and 11 days after seeding. .. DAPI Nucleic Acid staining (4′,6‐Diamidino‐2‐Phenylindole, Dihydrochloride, Invitrogen™, Carlsbad, California, USA, Catalog Number: D1306, Staining Condition: 1 : 1000 DAPI stock solution 2.5 mg·mL −1 , 10 min directly in cell culture medium) was performed for the not‐fixed spheroids on the last day of the experiment. ..

    Flow Cytometry:

    Article Title: Persistent glycolysis defines the foreign body response to polymeric implants
    Article Snippet: .. 0.5M EDTA (pH 08.0 ± 0.1; Corning 46-034-CI) 1M HEPES (Gibco 15630-080) 1X Antibody Diluent/Block (Akoya Biosciences ARD1001EA) 1X DPBS (Corning 20-031-CV) 1X OPAL TM Polymer HRP MS+RB (Akoya Biosciences ARH1001EA) 1X RPMI 1640 + L-Glutamine (300 mgL - ) (Corning 10-040-CV) 1X Plus Manual Amplification Diluent (Akoya Biosciences FP1498) 10% Neutral Buffered Formalin (VWR 16004-128) Alexa Fluor® 488 Conjugation Kit (Fast) – Lightning-Link ® (ABCAM AB236552) Alexa Fluor® 700 Conjugation Kit (Fast) – Lightning-Link ® (ABCAM AB269824) AR6 Antigen Retrieval Buffer (1-5% w/w Sodium Citrate; Akoya Biosciences AR600250ML) AR9 Antigen Retrieval Buffer (1-2.5% TRIS w/w, <1% EDTA w/w; Akoya Biosciences AR900259ML) BD Horizon TM Fixable Viability Stain 575V (BD Biosciences 565694) BSA (Heat shock fraction; Sigma-Aldrich A7906-50G) Collagenase A (Millipore-Sigma 10103586001) Chloroform (Fisher Scientific AC423555000) DAPI (1 mg mL −1 stock solution; Thermo Fisher Scientific D1306) DMSO (Sigma-Aldrich 67-68-5) DNase I (Millipore-Sigma 10104159001) DyLight® 405 Conjugation Kit (Fast) – Lightning-Link ® (ABCAM AB201798) Ethanol (Commercial Alchohols, P016EAAN) FBS (Heat inactivated, Corning 35-011-CV) FoxP3 Fixation/Permeabilization Buffer (BioLegend 421403) FoxP3 Permeabilization Solution (BioLegend 421403) Harringtonine (ABCAM AB141941) Histoplast TM PE (Thermo Fisher Scientific 22900700) Masson’s Trichrome Staining Kit (Polysciences Inc 25088-1) MitoTracker TM Red CMXRos Dye, for flow cytometry (Thermo Fisher Scientific M46752) Monocyte Blocker (BioLegend 426102) Mowiol® 4-88 (Fisher Scientific 9002-89-5) Oligomycin-A (ABCAM AB143423) Omni International 1.4 mm Ceramic Bead (Cole-Parmer® 19-645-3) PE-Cy7® Conjugation Kit – Lightning-Link ® (ABCAM AB102903) PE-Texas Red Conjugation Kit – Lightning-Link ® (ABCAM AB269899) Permount® Mounting Medium (Fisher Chemical SP15-500) Polydimethylsiloxane (PDMS; SYLGARD 184; Dow Chemical GMID 04019862) Polypropylene (PP; isotactic, avg. .. MW 220 000; Polysciences Inc. 06536) Puromycin (Sigma-Aldrich P8833) Precision Count Beads TM (BioLegend 424902) Rainbow Calibration Particles, 8 peaks (3.0-3.4 μm, BioLegend 422903) RNEasy Minikit Plus (Qiagen 74004) SsoAdvanced Universal SYBR Green Supermix (Bio-Rad 1725274) SuperScript IV VILO Master Mix (Thermo Fisher Scientific 11756050) Tris-HCl Buffered Saline + Tween®20 (TBST; 25 mM TRIS-HCl (pH 7.5), 150 mM NaCl, 0.05% Tween®20) TRIzol TM Reagent (Thiocyanic acid 25-40% w/w, Phenol 30-60% w/w, Ammonium thiocyanate 7-13% w/w; Invitrogen 15596018) TruStain FcX (BioLegend 101319) Ultracomp eBeads TM (Thermo Fisher Scientific, 01222242) Ultra-high MW polyethylene (UHMW-PE; avg.

    Cell Culture:

    Article Title: Obesity alters the fitness of peritumoral adipose tissue, exacerbating tumor invasiveness in renal cancer through the induction of ADAM12 and CYP1B1
    Article Snippet: The Leica DMi8 microscope digital camera system and the Leica Application Suite LAS X Imaging Software (Leica Microsystems GmbH) were used to measure the diameter of the spheroids following 2, 4, 7, 9, and 11 days after seeding. .. DAPI Nucleic Acid staining (4′,6‐Diamidino‐2‐Phenylindole, Dihydrochloride, Invitrogen™, Carlsbad, California, USA, Catalog Number: D1306, Staining Condition: 1 : 1000 DAPI stock solution 2.5 mg·mL −1 , 10 min directly in cell culture medium) was performed for the not‐fixed spheroids on the last day of the experiment. ..



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    90
    Millipore 1:1,000 dilution of diamidino-phenylindole (dapi) (stock solution 1 mg/ml)

    1:1,000 Dilution Of Diamidino Phenylindole (Dapi) (Stock Solution 1 Mg/Ml), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+solution(1+mg/1+1+000+dilution+of+diamidino+phenylindole++dapi+++stock+solution+1+mg+ml+/pmc03440724-80-12-18
    Average 90 stars, based on 1 article reviews
    1:1,000 dilution of diamidino-phenylindole (dapi) (stock solution 1 mg/ml) - by Bioz Stars, 2026-09
    90/100 stars
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    Journal: eLife

    Article Title: FABP4-mediated lipid accumulation and lipolysis in tumor-associated macrophages promote breast cancer metastasis

    doi: 10.7554/eLife.101221

    Figure Lengend Snippet:

    Article Snippet: Chemical compound, drug , DAPI solution (1 mg/mL) , Invitrogen , Cat#62248 , 1:1000.

    Techniques: Concentration Assay, Staining, Saline, SYBR Green Assay, Red Blood Cell Lysis, Cell Culture, XF Assay, Reverse Transcription, Polymer, Plasmid Preparation, Blocking Assay, Enzyme-linked Immunosorbent Assay, Sequencing, Transfection, Recombinant, Microarray, Expressing, Derivative Assay, Real-time Polymerase Chain Reaction, Software, Sterility, Migration, Pore Size, Flow Cytometry, Quantitative RT-PCR, Imaging, Microscopy, Transmission Assay, Electron Microscopy

    Journal: PLOS Pathogens

    Article Title: Pregnancy trained decidual NK cells protect pregnancies from harmful Fusobacterium nucleatum infection

    doi: 10.1371/journal.ppat.1011923

    Figure Lengend Snippet:

    Article Snippet: DAPI Solution (1 mg/mL) / 1 mL , Sigma-Aldrich , TS-62248.

    Techniques: Purification, Virus, Recombinant